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KMID : 0616120220530030125
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 3 p.125 ~ p.132
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Anti-oxidative and Anti-inflammatory Constituents from the Extracts of Brassica napus L. Whole Plant
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Jo Yeon-Jeong
Hyun Ju-Mi
Kang Ji-Mi
Kim Chang-Yun
Lee Nam-Ho
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Abstract
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In this study, we investigated anti-oxidative and anti-inflammatory efficacy, and identified their constituents from Brassica napus L. (Korean name: Yuchae) whole plant. Upon the anti-oxidative activities screening, the ethanol extract exhibited potent DPPH and ABTS+ radical scavenging activities. On the anti-inflammation studies using LPS-induced RAW264.7 cells, the extract inhibited the production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) effectively. To identify major constituents of B. napus extract, further purification was performed and led to isolation of two compounds; isorhamnetin 3,7-O-diglucoside(1) and isorhamnetin 3-O-glucoside(2). Quantitative analysis by high pressure liquid chromatography (HPLC) determined the flavonoid 1 as the major constituent. Isolated compounds showed DPPH radical scavenging effects and decreased NO levels without causing cell toxicities. These results indicate that the extract of Yuchae, a rich plant resource in Jeju Island, could be potentially applicable as an anti-oxidative and/or anti-inflammatory ingredients.
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KEYWORD
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Brassica napus L., Anti-oxidation, Anti-inflammation, Isolation
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